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piezo1 tdtomato reporter mice  (Jackson Laboratory)
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Jackson Laboratory piezo1 tdtomato reporter mice
Confocal microscopy of jejunal circular smooth muscle <t>from</t> <t>Piezo1-tdTomato</t> mice. (A) Left to right: Piezo1-tdTomato (cyan), RyR (magenta), merge with insets showing colocalization. Scale bars: 10 μm; inset 2 μm. (B,C) Imaris 3D surface classification separating membrane-localized (B: Piezo1 blue, RyR red) from intracellular (C: Piezo1 cyan, RyR magenta) signal (B,C) Imaris 3D surface classification separating membrane-localized (B: Piezo1 blue, RyR red) from intracellular (C: Piezo1 cyan, RyR2 magenta) signal. (D) Merged view showing extensive intracellular colocalization between Piezo1 (cyan) and RyR (magenta). (E–H) Quantification of Piezo1/RyR cluster distribution and association for n=50 cells from N=10 animals: Percentage of Piezo1 (E) and RyR (F) clusters localized at the plasma membrane (± 2 voxels) or intracellular compartments; the number (G) and percentage (H) of all Piezo1 clusters colocalized with RyR, either at the plasma membrane or intracellularly. Statistics: P-values as indicated above with p<0.05 marked as significant. data analyzed from n = 48 cells from N = 10 mice (5M/5F). No sex differences detected (all P > 0.6). Nested t-tests/nested two-way ANOVA ( Full statistics in ). Translucent symbols: individual cells; opaque: animal averages. Data: mean ± SD. Statistics: nested one-way ANOVA (K,L) or nested t-test, n = 127 cells from N = 16 mice. (I,J) Proximity ligation assay (PLA) detecting nanoscale interactions (<40 nm proximity). Max-intensity projections show PLA puncta (red) with Dapi (blue) (I) RyR-Piezo1 and (J) BK Ca -Piezo1. (K-M) Quantification (puncta per mm2) of (K) RyR-Piezo1 with 120.5 +/- 105.30 (L) BK Ca -Piezo1 67.57+/-39.85 and (M) a direct comparison between the two. n = 127 cells from N = 16 mice. Translucent symbols: individual cells; opaque: animal averages. Data: mean ± SD. Statistics: nested one-way ANOVA (K,L) or nested t-test (M) accounting for within-animal clustering. Single antibody controls verify PLA specificity.
Piezo1 Tdtomato Reporter Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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piezo1 tdtomato reporter mice - by Bioz Stars, 2026-08
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Confocal microscopy of jejunal circular smooth muscle from Piezo1-tdTomato mice. (A) Left to right: Piezo1-tdTomato (cyan), RyR (magenta), merge with insets showing colocalization. Scale bars: 10 μm; inset 2 μm. (B,C) Imaris 3D surface classification separating membrane-localized (B: Piezo1 blue, RyR red) from intracellular (C: Piezo1 cyan, RyR magenta) signal (B,C) Imaris 3D surface classification separating membrane-localized (B: Piezo1 blue, RyR red) from intracellular (C: Piezo1 cyan, RyR2 magenta) signal. (D) Merged view showing extensive intracellular colocalization between Piezo1 (cyan) and RyR (magenta). (E–H) Quantification of Piezo1/RyR cluster distribution and association for n=50 cells from N=10 animals: Percentage of Piezo1 (E) and RyR (F) clusters localized at the plasma membrane (± 2 voxels) or intracellular compartments; the number (G) and percentage (H) of all Piezo1 clusters colocalized with RyR, either at the plasma membrane or intracellularly. Statistics: P-values as indicated above with p<0.05 marked as significant. data analyzed from n = 48 cells from N = 10 mice (5M/5F). No sex differences detected (all P > 0.6). Nested t-tests/nested two-way ANOVA ( Full statistics in ). Translucent symbols: individual cells; opaque: animal averages. Data: mean ± SD. Statistics: nested one-way ANOVA (K,L) or nested t-test, n = 127 cells from N = 16 mice. (I,J) Proximity ligation assay (PLA) detecting nanoscale interactions (<40 nm proximity). Max-intensity projections show PLA puncta (red) with Dapi (blue) (I) RyR-Piezo1 and (J) BK Ca -Piezo1. (K-M) Quantification (puncta per mm2) of (K) RyR-Piezo1 with 120.5 +/- 105.30 (L) BK Ca -Piezo1 67.57+/-39.85 and (M) a direct comparison between the two. n = 127 cells from N = 16 mice. Translucent symbols: individual cells; opaque: animal averages. Data: mean ± SD. Statistics: nested one-way ANOVA (K,L) or nested t-test (M) accounting for within-animal clustering. Single antibody controls verify PLA specificity.

Journal: bioRxiv

Article Title: Intracellular Mechanosensation in Intestinal Smooth Muscle: Piezo1 Complexes Amplify Signaling Beyond the Surface

doi: 10.64898/2026.02.19.706403

Figure Lengend Snippet: Confocal microscopy of jejunal circular smooth muscle from Piezo1-tdTomato mice. (A) Left to right: Piezo1-tdTomato (cyan), RyR (magenta), merge with insets showing colocalization. Scale bars: 10 μm; inset 2 μm. (B,C) Imaris 3D surface classification separating membrane-localized (B: Piezo1 blue, RyR red) from intracellular (C: Piezo1 cyan, RyR magenta) signal (B,C) Imaris 3D surface classification separating membrane-localized (B: Piezo1 blue, RyR red) from intracellular (C: Piezo1 cyan, RyR2 magenta) signal. (D) Merged view showing extensive intracellular colocalization between Piezo1 (cyan) and RyR (magenta). (E–H) Quantification of Piezo1/RyR cluster distribution and association for n=50 cells from N=10 animals: Percentage of Piezo1 (E) and RyR (F) clusters localized at the plasma membrane (± 2 voxels) or intracellular compartments; the number (G) and percentage (H) of all Piezo1 clusters colocalized with RyR, either at the plasma membrane or intracellularly. Statistics: P-values as indicated above with p<0.05 marked as significant. data analyzed from n = 48 cells from N = 10 mice (5M/5F). No sex differences detected (all P > 0.6). Nested t-tests/nested two-way ANOVA ( Full statistics in ). Translucent symbols: individual cells; opaque: animal averages. Data: mean ± SD. Statistics: nested one-way ANOVA (K,L) or nested t-test, n = 127 cells from N = 16 mice. (I,J) Proximity ligation assay (PLA) detecting nanoscale interactions (<40 nm proximity). Max-intensity projections show PLA puncta (red) with Dapi (blue) (I) RyR-Piezo1 and (J) BK Ca -Piezo1. (K-M) Quantification (puncta per mm2) of (K) RyR-Piezo1 with 120.5 +/- 105.30 (L) BK Ca -Piezo1 67.57+/-39.85 and (M) a direct comparison between the two. n = 127 cells from N = 16 mice. Translucent symbols: individual cells; opaque: animal averages. Data: mean ± SD. Statistics: nested one-way ANOVA (K,L) or nested t-test (M) accounting for within-animal clustering. Single antibody controls verify PLA specificity.

Article Snippet: For immunocytochemistry experiments, Piezo1-tdTomato reporter mice (Piezo1tm1.1(KOMP), Jackson Laboratory Stock #029214; RRID: IMSR_JAX:029214) were bred and maintained as described previously ( Ranade et al ., 2014 ).

Techniques: Confocal Microscopy, Membrane, Clinical Proteomics, Proximity Ligation Assay, Comparison